Journal article
Transcriptomic Signatures of Human Immunodeficiency Virus Post-Treatment Control
A Wedrychowski, HA Martin, Y Li, S Telwatte, GN Kadiyala, M Melberg, B Etemad, E Connick, JM Jacobson, DM Margolis, D Skiest, P Volberding, F Hecht, S Deeks, JK Wong, JZ Li, SA Yukl
Journal of Virology | Published : 2023
DOI: 10.1128/jvi.01254-22
Abstract
Posttreatment controllers (PTCs) are rare HIV-infected individuals who can limit viral rebound after antiretroviral therapy interruption (ATI), but the mechanisms of this remain unclear. To investigate these mechanisms, we quantified various HIV RNA transcripts (via reverse transcription droplet digital PCR [RT-ddPCR]) and cellular transcriptomes (via RNA-seq) in blood cells from PTCs and noncontrollers (NCs) before and two time points after ATI. HIV transcription initiation did not significantly increase after ATI in PTCs or in NCs, whereas completed HIV transcripts increased at early ATI in both groups and multiply-spliced HIV transcripts increased only in NCs. Compared to NCs, PTCs showed..
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Grants
Awarded by National Institutes of Health
Funding Acknowledgements
We thank the study participants for their generous donations of samples, the site staff and investigators of the AIDS Clinical Trial Group, and the SCOPE project staff at Zuckerberg San Francisco General Hospital. In addition, we thank Zach Herbert and his group from the Molecular Biology Core Facilities (MBCF) at the Dana-Farber Cancer Institute (DFCI) and the Advanced Lab Technologies Core from the Harvard University Center for AIDS Research in Boston, MA for their assistance with the RNA-seq. This work was supported by the National Institute of Allergy and Infectious Diseases (R01150396 [to J.Z.L. and S.A.Y.], R01AI132128 [to S.A.Y. and J.K.W.], UM1AI068634 [to AIDS Clinical Trials Group (ACTG)], UM1AI068636 [to ACTG], UM1AI106701 [to ACTG and J.Z.L.], and 1P01AI169606 [to S.A.Y.]) as well as the National Institute of Diabetes and Digestive and Kidney Diseases (R01DK108349 [to S.A.Y.] and R01DK120387 [to S.A.Y.]). S.T. is supported by a CFAR Mentored Scientist in HIV Award (grant number P30 7AI027763, award number A120163, PI: P.V.) as well as the California HIV/AIDS Research Program (award number BB19-SF-009/A135087). Y.L was supported by an NIH T32 training grant (5T32AI007387-32).